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MT000810

UniProt Annotations

Entry Information

Gene Name	CD3e molecule, epsilon associated protein
Protein Entry	RPA34_HUMAN
UniProt ID	O15446
Species	Human

Comments

Comment type	Description
Alternative Products	Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=O15446-1; Sequence=Displayed; Name=2; Synonyms=CAST; IsoId=O15446-2; Sequence=VSP_017673; Note=No experimental confirmation available. Has sharply different functional characteristics.;
Caution	It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling. {ECO:0000305}.
Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
Function	Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
Miscellaneous	It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse, suggesting an important biological function.
Ptm	Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
Ptm	Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other groups.
Similarity	Belongs to the eukaryotic RPA34 RNA polymerase subunit family. {ECO:0000305}.
Subcellular Location	Nucleus, nucleolus {ECO:0000269\|PubMed:9426281}. Chromosome {ECO:0000269\|PubMed:9426281}. Note=Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
Subunit	Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E. {ECO:0000250, ECO:0000269\|PubMed:10373416, ECO:0000269\|PubMed:16809778}.