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MT000823

UniProt Annotations

Entry Information

Gene Name	acyl-CoA thioesterase 2
Protein Entry	ACOT2_HUMAN
UniProt ID	P49753
Species	Human

Comments

Comment type	Description
Alternative Products	Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P49753-1; Sequence=Displayed; Name=2; IsoId=P49753-2; Sequence=VSP_012225, VSP_012226;
Biophysicochemical Properties	Kinetic parameters: KM=40.3 uM for C10-acyl-CoA {ECO:0000269\|PubMed:16940157}; KM=8.9 uM for C12-acyl-CoA {ECO:0000269\|PubMed:16940157}; KM=1.6 uM for C14-acyl-CoA {ECO:0000269\|PubMed:16940157}; KM=2.0 uM for C16-acyl-CoA {ECO:0000269\|PubMed:16940157}; KM=2.8 uM for C18-acyl-CoA {ECO:0000269\|PubMed:16940157}; KM=4.8 uM for C20-acyl-CoA {ECO:0000269\|PubMed:16940157}; KM=4.5 uM for C16:1-acyl-CoA {ECO:0000269\|PubMed:16940157}; KM=6.1 uM for C18:1-acyl-CoA {ECO:0000269\|PubMed:16940157}; KM=4.3 uM for C18:1-trans-acyl-CoA {ECO:0000269\|PubMed:16940157}; Vmax=212 nmol/min/mg enzyme toward C10-acyl-CoA {ECO:0000269\|PubMed:16940157}; Vmax=681 nmol/min/mg enzyme toward C12-acyl-CoA {ECO:0000269\|PubMed:16940157}; Vmax=766 nmol/min/mg enzyme toward C14-acyl-CoA {ECO:0000269\|PubMed:16940157}; Vmax=656 nmol/min/mg enzyme toward C16-acyl-CoA {ECO:0000269\|PubMed:16940157}; Vmax=488 nmol/min/mg enzyme toward C18-acyl-CoA {ECO:0000269\|PubMed:16940157}; Vmax=408 nmol/min/mg enzyme toward C20-acyl-CoA {ECO:0000269\|PubMed:16940157}; Vmax=661 nmol/min/mg enzyme toward C16:1-acyl-CoA {ECO:0000269\|PubMed:16940157}; Vmax=304 nmol/min/mg enzyme toward C18:1-acyl-CoA {ECO:0000269\|PubMed:16940157}; Vmax=418 nmol/min/mg enzyme toward C18:1-trans-acyl-CoA {ECO:0000269\|PubMed:16940157};
Catalytic Activity	Palmitoyl-CoA + H(2)O = CoA + palmitate.
Caution	Was originally (PubMed:10944470) thought to be peroxisomal but was later shown (PubMed:16940157) to be mitochondrial. {ECO:0000305\|PubMed:10944470, ECO:0000305\|PubMed:16940157}.
Function	Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs. {ECO:0000269\|PubMed:10944470}.
Sequence Caution	Sequence=AAC42007.1; Type=Frameshift; Positions=215, 226; Evidence={ECO:0000305};
Similarity	Belongs to the C/M/P thioester hydrolase family. {ECO:0000305}.
Subcellular Location	Mitochondrion {ECO:0000269\|PubMed:16940157}.
Tissue Specificity	Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas. {ECO:0000269\|PubMed:16940157}.