MitoProteome Database

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MT001528

UniProt Annotations

Entry Information

Gene Name	cytidine monophosphate (UMP-CMP) kinase 2, mitochondrial
Protein Entry	CMPK2_HUMAN
UniProt ID	Q5EBM0
Species	Human

Comments

Comment type	Description
Alternative Products	Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=Q5EBM0-1; Sequence=Displayed; Name=2; IsoId=Q5EBM0-2; Sequence=VSP_033238, VSP_033239; Note=No experimental confirmation available.; Name=3; IsoId=Q5EBM0-3; Sequence=VSP_033240; Note=No experimental confirmation available.; Name=4; IsoId=Q5EBM0-4; Sequence=VSP_033241; Note=No experimental confirmation available.;
Biophysicochemical Properties	Kinetic parameters: KM=3.09 mM for CMP; KM=6.3 mM for UMP; KM=1.31 mM for dCMP; KM=0.1 mM for dUMP; Vmax=1.64 umol/min/mg enzyme towards CMP; Vmax=0.19 umol/min/mg enzyme towards UMP; Vmax=1.77 umol/min/mg enzyme towards dCMP; Vmax=0.48 umol/min/mg enzyme towards dUMP;
Catalytic Activity	ATP + (d)CMP = ADP + (d)CDP. {ECO:0000269\|PubMed:23416111}.
Catalytic Activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. {ECO:0000269\|PubMed:23416111}.
Function	May participate in dUTP and dCTP synthesis in mitochondria. Is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. May be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs. Also displays broad nucleoside diphosphate kinase activity. {ECO:0000269\|PubMed:17999954, ECO:0000269\|PubMed:23416111}.
Similarity	Belongs to the thymidylate kinase family. {ECO:0000305}.
Subcellular Location	Mitochondrion {ECO:0000269\|PubMed:17999954}.
Tissue Specificity	Among all investigated tumors, leukemia cells show the most abundant expression. {ECO:0000269\|PubMed:17999954}.