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MT000020

UniProt Annotations

Entry Information

Gene Name	adenylate kinase 4
Protein Entry	KAD4_HUMAN
UniProt ID	P27144
Species	Human

Comments

Comment type	Description
Biophysicochemical Properties	Kinetic parameters: KM=5.3 uM for AMP with ATP as phosphate donor {ECO:0000269\|PubMed:19766732}; KM=1.4 uM for AMP with GTP as phosphate donor {ECO:0000269\|PubMed:19766732}; KM=507 uM for dAMP with ATP as phosphate donor {ECO:0000269\|PubMed:19766732}; Vmax=90 pmol/min/ug enzyme with AMP as substrate and ATP as phosphate donor {ECO:0000269\|PubMed:19766732}; Vmax=80 pmol/min/ug enzyme with AMP as substrate and GTP as phosphate donor {ECO:0000269\|PubMed:19766732}; Vmax=88 pmol/min/ug enzyme with dAMP as substrate and ATP as phosphate donor {ECO:0000269\|PubMed:19766732};
Catalytic Activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. {ECO:0000255\|HAMAP-Rule:MF_03170}.
Catalytic Activity	NTP + AMP = NDP + ADP. {ECO:0000255\|HAMAP- Rule:MF_03170}.
Domain	Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP/ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP/ATP hydrolysis.
Function	Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity. {ECO:0000255\|HAMAP-Rule:MF_03170, ECO:0000269\|PubMed:19073142, ECO:0000269\|PubMed:19766732, ECO:0000269\|PubMed:23416111}.
Similarity	Belongs to the adenylate kinase family. AK3 subfamily. {ECO:0000255\|HAMAP-Rule:MF_03170}.
Subcellular Location	Mitochondrion matrix {ECO:0000255\|HAMAP- Rule:MF_03170, ECO:0000269\|PubMed:11485571, ECO:0000269\|PubMed:19766732}.
Subunit	Monomer. {ECO:0000255\|HAMAP-Rule:MF_03170, ECO:0000269\|Ref.11}.
Tissue Specificity	Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain. {ECO:0000269\|PubMed:11485571}.