MitoProteome Database

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MT001435

UniProt Annotations

Entry Information

Gene Name	autophagy related 4C, cysteine peptidase
Protein Entry	ATG4C_HUMAN
UniProt ID	Q96DT6
Species	Human

Comments

Comment type	Description
Biophysicochemical Properties	Kinetic parameters: KM=47.5 uM for MAP1LC3B {ECO:0000269\|PubMed:21177865}; KM=12.4 uM for GABARAPL2 {ECO:0000269\|PubMed:21177865};
Enzyme Regulation	Inhibited by N-ethylmaleimide.
Function	Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. {ECO:0000250, ECO:0000269\|PubMed:21177865}.
Sequence Caution	Sequence=BAB55356.1; Type=Frameshift; Positions=420; Evidence={ECO:0000305};
Similarity	Belongs to the peptidase C54 family. {ECO:0000305}.
Subcellular Location	Cytoplasm {ECO:0000250}.
Tissue Specificity	Highly expressed in skeletal muscle, heart, liver and testis. {ECO:0000269\|PubMed:12446702}.