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MT000128

UniProt Annotations

Entry Information
Gene Namecatechol-O-methyltransferase
Protein EntryCOMT_HUMAN
UniProt IDP21964
SpeciesHuman
Comments
Comment typeDescription
Alternative Products Event=Alternative initiation; Named isoforms=2; Name=Membrane-bound; Synonyms=MB-COMT; IsoId=P21964-1; Sequence=Displayed; Name=Soluble; Synonyms=S-COMT; IsoId=P21964-2; Sequence=VSP_018778;
Catalytic Activity S-adenosyl-L-methionine + a catechol = S- adenosyl-L-homocysteine + a guaiacol.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
Function Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Interaction Q15645:TRIP13; NbExp=3; IntAct=EBI-372265, EBI-358993;
Mass Spectrometry Mass=24352; Mass_error=2; Method=Electrospray; Range=52-271; Evidence={ECO:0000269|PubMed:8020475};
Polymorphism Low enzyme activity alleles are associated with genetic susceptibility to alcoholism [MIM:103780].
Polymorphism Two alleles, COMT*1 or COMT*H with Val-158 and COMT*2 or COMT*L with Met-158 are responsible for a three to four- fold difference in enzymatic activity.
Ptm The N-terminus is blocked.
Sequence Caution Sequence=AAH00419.2; Type=Erroneous termination; Positions=85; Note=Translated as Gln.; Evidence={ECO:0000305}; Sequence=AAH05867.1; Type=Erroneous termination; Positions=85; Note=Translated as Gln.; Evidence={ECO:0000305}; Sequence=ACI46037.1; Type=Erroneous termination; Positions=85; Note=Translated as Gln.; Evidence={ECO:0000305};
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O- methyltransferase family. {ECO:0000255|PROSITE-ProRule:PRU01019}.
Subcellular Location Isoform Membrane-bound: Cell membrane; Single-pass type II membrane protein; Extracellular side.
Subcellular Location Isoform Soluble: Cytoplasm.
Tissue Specificity Brain, liver, placenta, lymphocytes and erythrocytes.
Web Resource Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/comt/";
Web Resource Name=Wikipedia; Note=Catechol-O-methyl transferase entry; URL="http://en.wikipedia.org/wiki/Catechol-O-methyl_transferase";